Reporter

Part:BBa_K2429033:Design

Designed by: Nia Myrie   Group: iGEM17_MIT   (2017-10-24)


phEF1a mKate FF4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1722
    Illegal EcoRI site found at 2339
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1722
    Illegal EcoRI site found at 2339
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1722
    Illegal EcoRI site found at 2339
    Illegal BglII site found at 569
    Illegal BamHI site found at 1183
    Illegal XhoI site found at 968
    Illegal XhoI site found at 1619
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1722
    Illegal EcoRI site found at 2339
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1722
    Illegal EcoRI site found at 2339
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2244
    Illegal SapI.rc site found at 1237


Design Notes

Ensure that the intron can be efficient spliced out of the construct.



Source

Part received from Weiss Lab

References